Products
PIPES
Synonyms:1,4-piperazinediethane sulfonic acid;
1,4-piperazinediethanesulphonic acid;
1,4-piperazineethanesulfonate;piperazine-N,N'-bis(2-ethanesulfonic acid);
piperazine-N,N'-bis(2-ethanesulfonic acid), sodium salt;PIPES
Chemical Property of PIPES
● Appearance/Colour:White/clear crystalline powder
● Melting Point:>300 °C(lit.)
● Refractive Index:1.6300 (estimate)
● PKA:6.8(at 25℃)
● PSA：131.98000
● Density:1.505 g/cm3
● LogP:0.41700
● Storage Temp.:Store at RT.
● Solubility.:0.1 M NaOH: 0.25 g/mL, clear, colorless
● Water Solubility.:Soluble in water.
● XLogP3:-6.7
● Hydrogen Bond Donor Count:2
● Hydrogen Bond Acceptor Count:8
● Rotatable Bond Count:6
● Exact Mass:302.06062865
● Heavy Atom Count:18
● Complexity:397
Purity/Quality
99% *data from raw suppliers
1,4-Piperazinebis(ethanesulfonicacid) 99% *data from reagent suppliers
Safty Information
● Pictogram(s):
Xi
● Hazard Codes:Xi
● Statements:36/37/38
● Safety Statements:22-24/25-37/39-26
Useful
Chemical Classes:Other Uses -> Biological Buffers
Canonical SMILES:C1CN(CCN1CCS(=O)(=O)O)CCS(=O)(=O)O
Uses:Piperazine-N, N'-bis- (2-ethanesulphonic acid) ultra -pure >99.5%) is an important chemical intermedia in all kinds of chemical fields. 1,4-Piperazinediethanesulfonic acid, piperazine-N, N’-bis- (2-ethanesulfonic acid) (PIPES) is also one of the zwitterion buffers and the most appropriate buffer for the biological experiments using an intracellular solution since the Pk2 of Pipes is 6.8 at 20 degrees Celsius. A buffering agent with a pKa near physiological pH.PIPES [piperazine-N,N′-bis(2-ethanesulfonic acid)] is frequently used as a buffering agent in biochemistry. It is an ethanesulfonic acid buffer developed by Good et al. in the 1960s. PIPES has a pKa near the physiological pH which makes it useful in cell culture work. It has been documented to minimize lipid loss when buffering glutaraldehyde histology in plant and animal tissues.Additional forms available: PIPES, Sesquisodium Salt ; PIPES dipotassium salt; PIPES disodium salt; PIPES, Sodium Salt. Buffers can be prepared by adding a solution of base to PIPES free acid, titrating to the appropriate pH, or by mixing equimolar solutions of the monosodium salt and the disodium salt, titrating to the appropriate pH. PIPES, is used buffering agent in biochemistry. PIPES has pKa (6.76 at 25°C) near the physiological pH which makes it useful in cell culture work. Protocols have been reported on the use of PIPES for separation of glyoxylated RNA in agarose gels, nuclease S1 mapping of RNA, and in ribonuclease protection assay protocols. PIPES has been used as a buffer in glutaraldehyde fixation of tissue samples., PIPES has been utilized in protein crystallization., The use of PIPES in the reconstitution of dissociated tubulin α and β subunits after their resolution on immunoadsorbent gels has been described. PIPES has been recommended for use in buffers for the in vitro study of caspases 3, 6, 7, and 8. A published study demonstrated the usefulness of PIPES as a non-metal ion complexing buffer in such applications as protein assays. PIPES has been used in cell culture for such applications as the engineering of a thermostable mutant membrane protein in Escherichia coli.
Detailed Introduction
PIPES free acid, also known as 1,4-piperazinediethanesulfonic acid, is a Good's buffer notable for having a pKa value similar to ordinary physiological pH. As such, PIPES free acid is frequently used as a buffer in biochemical research.PIPES is a zwitterionic, piperazinic buffer that is useful for a pH range of 6.1 – 7.5. PIPES lacks the ability to form a significant complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions. PIPES has wide variety of applications and is commonly used in cell culture media, in protein crystallization, as a running buffer in gel electrophoresis, and as an eluent in isoelectric focusing and chromatography. This buffer is capable of forming radicals and is therefore not suitable for redox reactions. It is suitable for use in the bicinchoninic acid (BCA) assay. Solubility of PIPES increases when the free acid is converted to the sodium salt.Buffers are made by adding base solution to PIPES free acid and titrating to the desired pH
PIPES is a member of the ethanesulfonic acid buffer series, first introduced by Good et al., developed to meet certain criteria: midrange pKa, maximum water solubility and minimum solubility in all other solvents, minimal salt effects, minimal change in pKa with temperature, chemically and enzymatically stable, minimal absorption in visible or UV spectral range and easily synthesized. Since its pKa at 37 °C is near physiological pH, PIPES has applications in cell culture work.
Detailed Introduction
PIPES, also known as piperazine-N,N'-bis(2-ethanesulfonic acid), is a buffer commonly used in biological and biochemical research. It is a zwitterionic compound that helps maintain a stable pH in aqueous solutions.
One of the primary applications of PIPES is in the field of molecular biology and biochemistry, where it is utilized as a buffer for the preparation and storage of biological samples. PIPES has a pKa value of approximately 7.46, which makes it ideal for maintaining a pH range of 6.7 to 7.9 at physiological temperatures (around 37 degrees Celsius).
PIPES is particularly useful for maintaining a stable pH during processes such as protein purification, enzyme assays, and gel electrophoresis. It helps to minimize pH changes that could potentially affect the structure and function of proteins and enzymes.
Another application of PIPES is in cell culture research. It is commonly used as a buffer for cell culture media. PIPES helps to maintain a constant pH in the growth medium, which is crucial for the growth and viability of cells. It also provides a stable environment for studying cellular processes and conducting experiments.
PIPES is also utilized in various analytical techniques, such as liquid chromatography and capillary electrophoresis. Its buffering capacity and pH stability make it suitable for use in these techniques, where precise pH control is essential for accurate results.
Additionally, PIPES has found applications in drug formulation and delivery systems. It can be used as a buffer in pharmaceutical formulations to optimize drug stability and enhance drug absorption.
Overall, PIPES is a versatile buffering agent with a wide range of applications in biological and biochemical research, cell culture, analytical techniques, and pharmaceutical formulations. Its ability to maintain a stable pH makes it a valuable tool in various scientific fields.
