Products
MOPSO sodium salt
Synonyms:4-Morpholinepropanesulfonicacid, b-hydroxy-, monosodium salt (9CI)
Chemical Property of MOPSO Sodium Salt
● PKA:6.9(at 25℃)
● PSA：98.28000
● LogP:-0.75660
● Storage Temp.:Store at RT.
● Solubility.:H2O: 1 M at 20 °C, clear, colorless
Safty Information
● Pictogram(s):
Xi
● Hazard Codes:Xi
● Statements:36/37/38
● Safety Statements:26-36
Useful
Uses:MOPSO Sodium is a biological buffer also referred to as a second generation “Good′s” buffer which displays improved solubility compared to traditional “Good′s” buffers. The pKa of MOPSO Sodium is 6.9 which make it an ideal candidate for buffer formulations which require a pH slightly below physiological to maintain a stable environment in solution. MOPSO Sodium is considered to be non-toxic to culture cell lines and provides high-solution clarity.MOPSO Sodium can be used in cell culture media, biopharmaceutical buffer formulations (both upstream and downstream) and diagnostic reagents. MOPSO based buffers have been described for fixation of cells from urine samples.
Detailed Introduction
MOPSO sodium salt, also known as sodium 3-(N-morpholino)propanesulfonate, is a commonly used buffer in biological and biochemical research. It is a white crystalline powder that is very soluble in water. MOPSO sodium salt is often used as a buffer to maintain a stable pH value in various biological experiments and enzymatic reactions. It is especially useful for applications requiring a pH range of 6.5 to 7.9 because of its pKa value of 7.2. This buffer range makes it suitable for cell culture, protein purification, and molecular biology techniques.
In addition to its buffering capacity, MOPSO sodium salt also has the ability to stabilize certain proteins and enzymes, helping to maintain their activity and structure. It is considered a zwitterionic buffer, meaning it can exist in positively and negatively charged forms, depending on the pH of the solution. When using MOPSO sodium salt, it is important to measure and prepare buffer solutions accurately to achieve the desired pH level. A calibrated pH meter or pH indicator is recommended to monitor and adjust the pH accordingly.
Overall, MOPSO sodium salt is a valuable tool in laboratory research, providing a stable pH environment and supporting various biological and biochemical experiments.
Application
MOPSO Sodium Salt (3-(N-morpholino)propanesulfonic acid sodium salt) has several useful applications, particularly in the field of biochemistry and biotechnology. Here are some ways in which MOPSO Sodium Salt can be beneficial:
Buffering Agent: MOPSO Sodium Salt is commonly used as a buffering agent in various biological and biochemical experiments. It helps maintain a stable pH range, which is crucial for optimal enzyme activity, protein stability, and other biological processes.
Protein Crystallization: MOPSO Sodium Salt is often used in protein crystallization screens to facilitate the growth of high-quality protein crystals. Its buffering capacity helps to maintain a consistent pH, which is essential for the stability and structure of the protein crystals.
Electrophoresis: MOPSO Sodium Salt serves as a buffering component in techniques like SDS-PAGE (Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis) used for separating and analyzing proteins. Its buffering properties help maintain a stable pH, ensuring accurate protein separation and analysis.
Enzyme Assays: MOPSO Sodium Salt is useful in enzyme assays and kinetic studies, where it helps maintain a stable and controlled pH environment. This is crucial for accurate measurement of enzyme activity and understanding kinetic parameters.
Biochemical Solutions: MOPSO Sodium Salt is used in the formulation of biochemical solutions, such as buffers for protein extraction, purification, and storage. Its buffering capacity ensures that the pH remains constant, minimizing variations in protein behavior and functionality.
It's important to note that the specific concentration and conditions of MOPSO Sodium Salt usage can vary depending on the specific experiment or application. Always refer to the relevant protocols and guidelines for the appropriate preparation and usage of MOPSO Sodium Salt in your experiments.
