Products
MOPSO
Synonyms:3-morpholino-2-hydroxypropanesulfonic acid;3-morpholino-2HOPSA
Chemical Property of MOPSO
● Appearance/Colour:white crystalline powder
● Melting Point:275-280 °C (dec.)
● Refractive Index:1.539
● PKA:pK1:6.75 (37°C)
● PSA：95.45000
● Density:1.416 g/cm3
● LogP:-0.41400
● Storage Temp.:Store at RT.
● Solubility.:H2O: 0.5 M at 20 °C, clear
● Water Solubility.:Water solubility under desired conditions ca.112,6 g/L at 20°C.
● XLogP3:-4.1
● Hydrogen Bond Donor Count:2
● Hydrogen Bond Acceptor Count:6
● Rotatable Bond Count:4
● Exact Mass:225.06709375
● Heavy Atom Count:14
● Complexity:254
Safty Information
● Pictogram(s):
Xi
● Hazard Codes:Xi
● Statements:36/37/38
● Safety Statements:26-36-37/39
Useful
Chemical Classes:Nitrogen Compounds -> Morpholines
Canonical SMILES:C1COCCN1CC(CS(=O)(=O)O)O
Uses:MOPSO is a buffer that works in the 6-7 pH range. Used in pharmaceutical synthesis. MOPSO is a biological buffer also referred to as a second generation “Good′s” buffer which displays improved solubility compared to traditional “Good′s” buffers. The pKa of MOPSO is 6.9 which make it an ideal candidate for buffer formulations which require a pH slightly below physiological to maintain a stable environment in solution. MOPSO is considered to be non-toxic to culture cell lines and provides high-solution clarity.MOPSO can be used in cell culture media, biopharmaceutical buffer formulations (both upstream and downstream) and diagnostic reagents.
Detailed Introduction
MOPSO (3-(N-Morpholino)propanesulfonic acid) is a chemical compound that belongs to the class of sulfonic acids. Here are some key points about MOPSO:
Chemical Structure: MOPSO has the chemical formula C7H17NO4S and consists of a morpholine ring (a saturated heterocyclic compound) attached to a propane chain with a sulfonic acid group (SO3H).
Buffer Properties: MOPSO is commonly used as a buffer in biochemistry and molecular biology experiments. It has a pKa value of approximately 7.20 at 25°C, making it suitable for buffering within the pH range of 6.2 to 7.6.
Buffer Capacity: MOPSO has a moderate buffering capacity within its effective pH range. It can help to maintain a stable pH in various biological and chemical systems, preventing large fluctuations that may affect the activity of enzymes or the stability of molecules.
Biological Applications: MOPSO is often used in cell culture, protein purification, and other biological experiments that require a specific pH range. It is particularly useful in situations where the pH needs to be maintained close to physiological conditions.
Chemical Stability: MOPSO is chemically stable and does not readily degrade under typical storage conditions. However, it is important to store it in a dry and cool environment to prevent moisture absorption and potential degradation.
Safety Considerations: MOPSO is generally considered to be safe for use in laboratory settings when handled properly. However, as with any chemical, it is important to follow proper safety protocols, such as wearing appropriate protective equipment (gloves, goggles) and handling it in a well-ventilated area.
It is worth noting that the specific use and concentration of MOPSO may vary depending on the experimental requirements. Always refer to the product's technical data sheet or consult with a knowledgeable professional for detailed instructions and considerations specific to your experiment.
Application
MOPSO has a wide range of applications in various scientific fields. Here are some common applications of MOPSO:
Buffering Agent: MOPSO is commonly used as a buffering agent in biological and biochemical research. It helps to maintain a constant pH in solutions, which is important for maintaining the activity and stability of enzymes, proteins, and other biomolecules.
Cell Culture: MOPSO is used in cell culture media to maintain a specific pH range (around pH 7.2) that is optimal for the growth and maintenance of certain cell lines.
Protein Purification: MOPSO can be used as a buffer in protein purification processes, where it helps to maintain the stability and activity of proteins during various purification steps.
Enzyme Studies: MOPSO is frequently used as a buffer in enzymatic studies to maintain an optimal pH for enzyme activity. It is particularly useful in studying enzymes that work optimally around pH 7.
Electrophoresis: MOPSO can be used as a buffer in electrophoresis techniques, such as SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis), to provide the desired pH range for protein separation and analysis.
Drug Formulation: MOPSO is used in the formulation of certain drugs, particularly those that require a specific pH range for stability and efficacy.
Chemical Synthesis: MOPSO can be employed as a reagent or catalyst in certain chemical reactions and synthesis processes.
It's important to note that the specific concentration and use of MOPSO may vary depending on the application and experimental requirements. Always consult the literature or follow the guidelines provided by manufacturers or researchers experienced in the specific field of study.
