Products
MOPS sodium salt
Synonyms:71119-22-7;MOPS sodium salt;Sodium 3-Morpholinopropanesulfonate;MOPS-Na;4-Morpholinepropanesulfonic acid, sodium salt;4-Morpholinepropanesulfonic acid sodium salt;MFCD00064350;3-(N-Morpholino)propanesulfonic acid sodium salt;MOPS (sodium salt);Sodium 3-morpholin-4-ylpropane-1-sulfonate;3-(4-Morpholino)propanesulfonic acid sodium salt;sodium 3-morpholinopropane-1-sulfonate;3-(4-Morpholino)propane sulfonic acid, Sodium Salt;sodium;3-morpholin-4-ylpropane-1-sulfonate;4-Morpholinepropanesulfonic acid, sodium salt (1:1);MOPS, Sodium;MOPS, sodium salt;sodium 3-(morpholin-4-yl)propane-1-sulfonate;C7H15NO4S.Na;SCHEMBL161682;DTXSID3072246;C7-H15-N-O4-S.Na;HY-D0859A;AKOS015897419;AKOS015964205;AKOS024306967;AC-24632;AS-14495;PD080188;SY061683;3-Morpholinopropanesulfonic Acid Sodium Salt;CS-0120956;FT-0613841;M0755;sodium 3-(4-morpholinyl)-1-propanesulfonate;EC 428-420-3;F20322;M-8501;3-(4-Morpholino)propane sulfonic acid,Sodium Salt;A837085
Chemical Property of MOPS Sodium Salt
● Appearance/Colour:white powder
● Melting Point:277-282 °C
● PKA:7.2(at 25℃)
● PSA：78.05000
● Density:1.41[at 20℃]
● LogP:0.27260
● Storage Temp.:Store at RT.
● Solubility.:H2O: 1 M at 20 °C, clear, colorless
● Water Solubility.:Soluble in water (523 g/L at 20°C).
● Hydrogen Bond Donor Count:0
● Hydrogen Bond Acceptor Count:5
● Rotatable Bond Count:4
● Exact Mass:231.05412338
● Heavy Atom Count:14
● Complexity:233
Safty Information
● Pictogram(s):
Xi
● Hazard Codes:Xi
● Statements:36/37/38
● Safety Statements:24/25-36-26
Useful
Canonical SMILES:C1COCCN1CCCS(=O)(=O)[O-].[Na+]
Uses:MOPS Sodium Salt is a buffering agent used in organic chemistry.
Detailed Introduction
MOPS sodium salt is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al.
It is a zwitterionic, morpholinic buffer that is useful for a pH range of 6.5 – 7.9 and commonly used for cell culture media, as a running buffer in electrophoresis and for protein purification in chromatography.
MOPS lacks the ability to form a complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions. MOPS is often used in buffered culture media for bacteria, yeast, and mammalian cells. MOPS is regarded as an excellent buffer for use in separating RNA in agarose gels. It is recommended to sterilize MOPS buffers by filtration rather than with autoclave due to the unknown identity of yellow degradation products that occur after sterilization of MOPS with autoclave. It is suitable for use in the bicinchoninic acid (BCA) assay.
MOPS sodium salt can be mixed with MOPS free acid to attain desired pH. Alternatively, MOPS free acid can be titrated with sodium hydroxide to attain desired pH.
Application
MOPS Sodium Salt, also known as 3-(N-morpholino)propanesulfonic acid sodium salt, is a buffering agent commonly used in biological research and molecular biology applications.It is a zwitterionic buffer that maintains a stable pH range and helps to maintain optimal conditions for enzymatic reactions and other biological processes.
One of the main applications of MOPS Sodium Salt is in cell culture and media formulations. It is often used as a buffering agent in cell culture media to maintain a constant pH and provide a stable environment for cell growth and proliferation. MOPS buffering is commonly used for mammalian cell culture systems.
MOPS Sodium Salt is also used in DNA and RNA gel electrophoresis. It functions as a running buffer to ensure a stable pH throughout the electrophoresis process. It helps to maintain the integrity and stability of nucleic acids during separation and analysis by gel electrophoresis.
Furthermore, MOPS Sodium Salt is utilized in protein research and analysis techniques, such as SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis). It is commonly used as a component of the sample buffer in protein sample preparation, helping to solubilize and denature proteins effectively.
In molecular biology applications, MOPS Sodium Salt is often used in PCR (polymerase chain reaction) and other DNA amplification techniques. Its buffering capacity helps to maintain the pH at an optimal range for the activity of DNA polymerases and other enzymes involved in the amplification process.
MOPS Sodium Salt can also be utilized as a buffer in various biochemical assays, enzyme kinetics studies, and protein purification processes. Its ability to maintain a stable pH range makes it valuable in maintaining the activity and stability of enzymes and proteins during these experimental procedures.
It is essential to follow the recommended guidelines for the preparation and use of MOPS Sodium Salt to ensure accurate results and reproducibility in experiments. Additionally, it is important to store the reagent properly and avoid contamination to maintain its effectiveness.
