Products
MES sodium salt
Synonyms:4-Morpholineethanesulfonicacid, sodium salt
(9CI);2-(N-Morpholino)ethanesulfonic acid sodium salt;
Sodium2-(N-morpholino)ethanesulfonate;
Chemical Property of MES sodium salt
● Appearance/Colour:White powder
● Vapor Pressure:0Pa at 25℃
● PSA：78.05000
● Density:1.507[at 20℃]
● LogP:-0.11750
● Storage Temp.:Store at RT.
● Solubility.:H2O: 0.5 g/mL, clear, colorless
● Water Solubility.:H2O: 0.5 g/mL, clear, colorless
Purity/Quality
99% *data from raw suppliers
2-(N-Morpholino)ethanesulfonicacidsodiumsalt 99% *data from reagent suppliers
Safty Information
● Pictogram(s):
Xi
● Hazard Codes:Xi
● Statements:36/37/38
Safety Statements:22-24/25-36-26
MSDS Files
Useful
UsesMES sodium salt is a buffering agent used in biological and biochemical research including plant cell culture. MES sodium salt has been used:To achieve the required density and stretching of DNA fibers during melting of agarose plugs containing genomic DNATo equilibrate the Balch homogenizer chamber and to prevent sample hydrolysis before sample homogenization MES Sodium is a biological buffer often referred to as a “Good′s” buffer. The pKa of MES is 5.96 which makes MES an ideal candidate for cell culture media and protein based buffer formulations to maintain a stable environment in solution. MES Sodium is considered to be non-toxic to culture cell lines, highly water soluble and provides high-solution clarity.MES Sodium is used in cell culture media, biopharmaceutical buffer formulations (both upstream and downstream) and diagnostic reagents. MES based buffers are used in purification bioprocesses of antibodies, peptides, proteins and blood components.
Key Points
MES-Na refers to the sodium salt form of 4-Morpholineethanesulfonic acid (MES). It is commonly used in biochemical research and molecular biology as a buffer in various applications. Here are some key points about MES-Na:
Sodium salt form: MES-Na is the sodium salt of MES, which means it is the form of MES that has been converted into its sodium salt by neutralizing the MES acid with sodium hydroxide or another sodium base.
Buffering properties: Like free MES, MES-Na is an effective buffering agent that helps maintain a constant pH in biological and chemical experiments. It provides the advantage of being in a salt form, allowing better solubility in water and easier handling compared to the free acid form.
Stability: MES-Na exhibits good stability at different temperatures, making it suitable for maintaining a stable pH range in various experimental conditions. It is less affected by temperature changes compared to other buffers like phosphate buffers.
Protein and enzyme studies: MES-Na is commonly used in protein purification, enzyme assays, and other biochemical experiments involving proteins and enzymes. Its buffering capacity and stability at physiological pH make it suitable for these applications.
Cell culture: Similar to free MES, MES-Na can also be used in cell culture media to help maintain a stable pH for the growth and maintenance of certain cell types.
Remember to follow the instructions provided by the manufacturer when working with MES-Na, including the appropriate concentration and pH for specific applications. Additionally, take the necessary precautions and safety measures when handling this compound, as it may cause irritation to the eyes, skin, and respiratory tract.
