Products
HEPPS
Synonyms:Hepps;16052-06-5;EPPS;4-(2-Hydroxyethyl)-1-piperazinepropanesulfonic acid;3-(4-(2-Hydroxyethyl)piperazin-1-yl)propane-1-sulfonic acid;3-[4-(2-Hydroxyethyl)-1-piperazinyl]propanesulfonic Acid;1-Piperazinepropanesulfonic acid, 4-(2-hydroxyethyl)-;3-[4-(2-HYDROXYETHYL)PIPERAZIN-1-YL]PROPANE-1-SULFONIC ACID;N-(2-Hydroxyethyl)piperazine-N'-(3-propanesulfonic acid);4-(2-Hydroxyethyl)piperazine-1-propanesulfonic acid;C9H20N2O4S;4-(2-Hydroxyethyl)-1-piperazinepropanesulphonic acid;EINECS 240-198-8;BRN 3957385;8JN5PQW99C;4-(2-Hydroxyethyl)-1-piperazinepropane sulfonic acid;MFCD00006160;3-[4-(2-Hydroxyethyl)-1-piperazinyl]-1-propanesulfonic acid;EP1;N-2-Hydroxyethylpiperazine-N'-3-propanesulfonic acid;UNII-8JN5PQW99C;SCHEMBL98490;DTXSID3066003;CHEBI:42298;EPPS, >=99.5% (titration);HY-D0873;C9-H20-N2-O4-S;BBL023080;MFCD12546632;STL356002;AKOS005267107;EPPS, BioUltra, >=99.0% (T);EPPS, Vetec(TM) reagent grade, 99%;AC-24630;AS-69273;EPPS, BioXtra, >=99.5% (titration);LS-113260;CS-0015183;FT-0616538;H0576;F20313;M01848;A810147;N-(2-Hydroxyethyl)-1-piperazinepropanesulfonic acid;N-(Hydroxyethyl)piperazine-N'-propane sulfonic acid;N-2-Hydroxyethylpiperazine-N-3-propanesulfonic acid;Q1563525;W-107976;3-[4-(2-Hydroxyethyl)-1-piperazinyl]propanesulfonicAcid;4-(2-Hydroxyethyl)-1-piperazine-3-propanesulfonic acid;4-(2-Hydroxyethyl)piperazine-1-propane-3-sulfonic acid;N-(2-Hydroxyethyl)piperazine-N'-(propanesulphonic acid);3-(4-(2-hydroxyethyl)piperazinyl)propanesulfonic acid (HEPPS);3-[4-(2-Hydroxyethyl)-1-piperazinyl]-1-propanesulfonic acid #;EPPS, BioPerformance Certified, cell culture tested, >=99.5% (titration);3-[4-(2-hydroxyethyl)piperazin-1-yl]propylsulfonyloxonium
Chemical Property of HEPPS
● Appearance/Colour:white crystalline powder
● Melting Point:237-239 °C(lit.)
● Refractive Index:1.537
● PKA:8.0(at 25℃)
● PSA：89.46000
● Density:1.285 g/cm3
● LogP:-0.16920
● Storage Temp.:Store at RT.
● Solubility.:H2O: 1 M at 20 °C, clear, colorless
● Water Solubility.:Soluble in water. 25.2 g/L (20°C)
● XLogP3:-3.6
● Hydrogen Bond Donor Count:2
● Hydrogen Bond Acceptor Count:6
● Rotatable Bond Count:6
● Exact Mass:252.11437830
● Heavy Atom Count:16
● Complexity:280
Safty Information
● Pictogram(s):
Xi
● Hazard Codes:Xi
● Statements:36/37/38-22
● Safety Statements:24/25-36-26
Useful
Chemical Classes:Nitrogen Compounds -> Piperazines
Canonical SMILES:C1CN(CCN1CCCS(=O)(=O)O)CCO
Uses:EPPS is a commonly used Good?s buffer. It is often used as separator in ultrathin isoelectric focusing gels and enhances the resolution of phosphoglucomutase. It is used as a buffering agent in biology and biochemistry.
Detailed Introduction
EPPS stands for N-(2-Hydroxyethyl)piperazine-N’-(3-propanesulfonic acid). It is a zwitterionic buffering agent that is commonly used in biological and biochemical research.Here are some key points about EPPS:
Buffering properties: EPPS is an effective buffering agent that helps maintain a constant pH in experimental setups. It has a pKa value of 8.09, which makes it suitable for buffering in the pH range of 7.0-9.0.
Stability: EPPS exhibits good stability over a wide range of temperatures and is less affected by temperature changes compared to other buffering agents like Tris and phosphate buffers. This stability makes it suitable for use in biochemical and biological experiments that require controlled pH conditions.
Biological applications: EPPS is commonly used in various biological studies, such as protein purification, enzyme assays, and DNA/RNA isolation. Its buffering capacity and stability at physiological pH make it valuable in these applications.
Cell culture: EPPS can also be used as a buffering agent in cell culture media. It helps maintain a stable pH environment conducive to the growth and maintenance of certain cell types.
Ions and compatibility: EPPS is a zwitterionic compound, meaning it contains both positive and negative charges. This feature allows it to effectively buffer both acidic and basic solutions. It is compatible with various biological molecules and is known to have minimal interference with enzymatic reactions.
When using EPPS, it is important to follow the recommended concentration and pH guidelines provided by the manufacturer. Additionally, always exercise caution and follow proper safety protocols when handling any chemical or buffering agent.
Application
HEPPS (N-(2-Hydroxyethyl)piperazine-N'-3-propanesulfonic acid) is a zwitterionic buffer that is commonly used in biological and biochemical research. Some of its applications include:
Cell Culture: HEPPS is used as a buffering agent in cell culture media to maintain a constant pH and provide a suitable environment for cell growth and proliferation. It helps stabilize the pH within the physiological range and avoid any adverse effects on cellular processes.
Enzyme Assays: HEPPS is often used as a buffer in enzyme assays to optimize the pH conditions required for maximum enzyme activity. Its buffering capacity helps maintain a stable pH, ensuring accurate and reliable enzyme measurements.
Protein Purification: HEPPS can be utilized as a buffer in protein purification processes. It helps maintain the stability and activity of proteins during purification steps, such as column chromatography or protein purification using affinity tags.
Electrophoresis: HEPPS is sometimes used as a buffer in gel electrophoresis for the separation and analysis of proteins and nucleic acids. Its pH buffering capacity helps maintain a stable pH during the electrophoretic run, aiding in accurate separation of molecules.
Molecular Biology Techniques: HEPPS can be used as a buffer in various molecular biology techniques, including PCR (Polymerase Chain Reaction), DNA sequencing, and enzymatic reactions. Its buffering capability ensures optimal pH conditions for successful and efficient reactions.
Studies Involving Membrane Proteins: HEPPS is well-suited for studying membrane proteins due to its low membrane-penetrating properties. It is often used as a buffer in experiments involving the functional characterization or reconstitution of membrane proteins.
It's important to note that the optimal concentration and pH of HEPPS may vary depending on the specific application or experimental conditions. Therefore, it is advisable to consult the literature or supplier recommendations for the appropriate use and concentration of HEPPS in your experiments.
