HEPES sodium salt

Short Description:

Chemical Name:HEPES sodium salt
CAS No.:75277-39-3
Deprecated CAS:1159813-57-6
Molecular Formula:C8H17N2NaO4S
Molecular Weight:260.29
Hs Code.:29335995
European Community (EC) Number:278-169-7,688-394-6
UNII:Z9FTO91O8A
DSSTox Substance ID:DTXSID4044458
Nikkaji Number:J307.551F
Wikidata:Q27120698
ChEMBL ID:CHEMBL3187284
Mol file:75277-39-3.mol

Product Detail

Product Tags

Synonyms:HEPES sodium salt;75277-39-3;HEPES hemisodium salt;103404-87-1;Sodium 2-(4-(2-hydroxyethyl)piperazin-1-yl)ethanesulfonate;sodium 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonate;HEPES (sodium);C8H17N2NaO4S;4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid hemisodium salt;1-Piperazineethanesulfonic acid, 4-(2-hydroxyethyl)-, monosodium salt;4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt;Sodium 4-(2-hydroxyethyl)piperazin-1-ylethanesulphonate;UNII-Z9FTO91O8A;Z9FTO91O8A;sodium;2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonate;DTXSID4044458;CHEBI:46758;EINECS 278-169-7;1-Piperazineethanesulfonic acid, 4-(2-hydroxyethyl)-, sodium salt (1:1);N-(2-Hydroxyethyl)piperazine-N'-2-ethanesulfonic acid,sodium salt;HEPES sodium salt, >=99.5% (titration);sodium hepes;N-(2-Hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid) sodium salt;MFCD00036463;HEPES, sodium salt;HEPES sodium salt, 98%;C8H18N2O4S.Na;SCHEMBL229142;WAS-14;CHEMBL3187284;DTXCID2024458;RDZTWEVXRGYCFV-UHFFFAOYSA-M;C8-H18-N2-O4-S.Na;HEPES sodium salt solution (1M);Tox21_302155;HB5187;N-(2-Hydroxyethyl)piperazine-N-(2-ethanesulfonicacid)hemisodiumsalt;HEPES sodium salt, biochemical grade;AKOS015897769;AKOS015912229;AKOS015964204;NCGC00255791-01;CAS-75277-39-3;HY-108535;CS-0029103;FT-0610868;F20319;A838366;sodium N'-hydroxyethyl-N-piperazineethanesulfonate;HEPES sodium salt, BioXtra, >=99.0% (titration);HEPES sodium salt, Vetec(TM) reagent grade, 96%;W-104397;Q27120698;Sodium2-(4-(2-hydroxyethyl)piperazin-1-yl)ethanesulfonate;4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid sodium salt;4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid, monosodium salt;N-(2-Hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid)sodiumsalt;N-2-Hydroxyethylpiperazine-N'-2-ethanesulphonic acid hemisodium salt;HEPES sodium salt, BioPerformance Certified, suitable for cell culture, >=99.5%

Chemical Property of HEPES Sodium Salt

● Appearance/Colour:white powder
● Vapor Pressure:0Pa at 25℃
● Melting Point:234 °C
● PKA:7.5(at 25℃)
● PSA：92.29000
● Density:1.504[at 20℃]
● LogP:-0.90190

● Storage Temp.:Store at RT.
● Sensitive.:Hygroscopic
● Solubility.:H2O: 1 M at 20 °C, clear, colorless
● Water Solubility.:It is soluble in water.
● Hydrogen Bond Donor Count:1
● Hydrogen Bond Acceptor Count:6
● Rotatable Bond Count:5
● Exact Mass:260.08067248
● Heavy Atom Count:16
● Complexity:272

Safty Information

● Pictogram(s):
● Hazard Codes:
● Safety Statements:22-24/25

Useful

Chemical Classes:Other Uses -> Biological Buffers
Canonical SMILES:C1CN(CCN1CCO)CCS(=O)(=O)[O-].[Na+]
Uses:HEPES sodium salt solution is suitable for cell culture studies. It may be used in the preparation of buffer solution of HEPES. It may be used as buffer during the phosphorylation assays in permeabilized fibroblasts. It may be used to compose a complex dialysis buffer, used in the determination of free thyroxin in serum by radioimmunoassay. HEPES sodium salt solution is suitable for use in cell culture studies. It may be used in the preparation of buffer solution of HEPES. It may be used as buffer during phosphorylation assays in permeabilized fibroblasts. It may be used to compose a complex dialysis buffer, used in the determination of free thyroxin in serum by radioimmunoassay.

Detailed Introduction

HEPES sodium salt, also known as 4-(2-hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt, is a commonly used form of HEPES. It is a zwitterionic organic compound that acts as a buffering agent in biological and biochemical research.
HEPES sodium salt is often used in cell culture media and biological buffers due to its ability to maintain a stable pH range around physiological conditions (pH 7.2 - 7.6). It is highly soluble in water and has a relatively low toxicity, making it suitable for various applications.
The sodium salt form of HEPES is preferred in many applications as it enhances solubility and stability, compared to the free acid form. It is commercially available as a white crystalline powder that can be easily dissolved in water to prepare working solutions.
Researchers commonly use HEPES sodium salt in molecular biology techniques, cell culture, enzyme assays, protein purification, and other biochemical experiments where pH control is crucial. Its buffering capacity and compatibility with biological systems make it a popular choice for maintaining optimal conditions for the activity and stability of various biological molecules.
Before using HEPES sodium salt in any specific application, it is essential to review the literature, consult the suppliers' recommendations, and optimize the concentration and pH range based on the specific experimental requirements.

Application

HEPES sodium salt finds widespread application in various biological and biochemical research areas. Some of its key uses include:
Cell Culture: HEPES sodium salt is commonly added to cell culture media to maintain a stable pH within the physiological range and provide a suitable environment for cells to grow and proliferate.
Buffering Agent: HEPES sodium salt is frequently employed as a buffering agent in biological buffers and solutions, including enzyme assays, protein purification, and molecular biology techniques. It helps maintain a constant pH by resisting changes in acidity or alkalinity.
Electrophoresis: HEPES sodium salt is used as a buffer in gel electrophoresis to maintain a stable pH during the separation of nucleic acids or proteins. It prevents shifts in pH that can affect the migration and separation patterns of molecules in the gel.
Protein Stability: HEPES sodium salt is added to protein solutions to stabilize their structure and maintain their activity. It helps maintain the optimal pH environment necessary for protein stability and functionality.
Enzyme Activity: HEPES sodium salt is used as a buffer in enzymatic reactions to maintain the desired pH for optimal enzyme activity. It helps ensure that enzymes function at their highest efficiency and accuracy.
Live Cell Imaging: HEPES sodium salt is often included in the imaging media for live cell imaging experiments. Its buffering capacity helps maintain the desired pH and prevents fluctuations that may affect the viability and fluorescence of the cells.
Molecular Biology Techniques: HEPES sodium salt is widely employed in various molecular biology techniques, including DNA or RNA isolation, PCR, DNA sequencing, and protein analysis. Its buffering capability allows for accurate and reliable results during these procedures.
It's important to note that the specific application and concentration of HEPES sodium salt may vary depending on the experimental requirements and the biological system under study. Therefore, it's advisable to consult the literature and supplier recommendations for optimal use.