Products
HEPES
Synonyms:1-[4-(2-Hydroxyethyl)-1-piperazinyl]ethane-2-sulfonicacid;2-[4-(2-Hydroxyethyl)piperazinyl]ethanesulfonic acid;4-(2-Hydroxyethyl)piperazine-1-(2-ethanesulfonic acid);N-(2-Hydroxyethyl)piperazine-N'-2-ethanesulfonic acid;N-(2-Hydroxyethyl)piperazine-N'-ethanesulfonic acid;NSC 166663;N'-(2-Hydroxyethyl)piperazine-N-(2-ethanesulfonic acid);N'-2-Hydroxyethylpiperazine-N-2-ethanesulfonic acid;TVZ 7;WAS 13;HEPES (4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid);1-Piperazineethanesulfonicacid, 4-(2-hydroxyethyl)-;
Chemical Property of HEPES
● Appearance/Colour:White crystalline powder
● Vapor Pressure:0Pa at 25℃
● Melting Point:234-238 °C
● Refractive Index:n20/D 1.339
● Boiling Point:408℃[at 101 325 Pa]
● PKA:7.5(at 25℃)
● PSA：89.46000
● Density:1.325 g/cm3
● LogP:-0.55930
● Storage Temp.:2-8°C
● Solubility.:H2O: 1 M at 20 °C, clear, colorless
● Water Solubility.:Soluble
● XLogP3:-4.1
● Hydrogen Bond Donor Count:2
● Hydrogen Bond Acceptor Count:5
● Rotatable Bond Count:4
● Exact Mass:238.09872823
● Heavy Atom Count:15
● Complexity:254
Safty Information
● Pictogram(s):
Xi
● Hazard Codes:Xi
● Statements:36/37/38
● Safety Statements:24/25-22-36-26
Useful
Canonical SMILES:C1CN(CC[NH+]1CCS(=O)(=O)[O-])CCO
Description:HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2 (pKa 7.55). It is typically used in cell culture at concentration between 5mM to 30 mM. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.
Uses:Non-toxic to cell. It is used as a hydrogen ion buffer, which can control the constant pH range for long term. The concentration is 10-50mmol/L. Generally, 20mmol/LHEPES in nutrient solution can get buffer ability. Biological buffer. HEPES is common buffer for biological sciences, particularly used in cell culture to maintain physiological pH. It acts a buffering component, which is used in the preparation of buffers. It is described as one of the best all-purpose buffers available for use in biological research.HEPES has been used as a component of:Hank′s balanced salt solution, Dulbecco′s modified eagle′s medium and no-glucose DMEM, which are used for the preparation of tissue slices.Buffers used in localized conductance measurements on the basolateral side of culture inserts to examine cation selectivity.
Detailed Introduction
HEPES stands for 2-(4-(2-hydroxyethyl)piperazin-1-yl)ethanesulfonic acid. It is a buffering agent commonly used in biological and biochemical research. HEPES helps maintain a stable pH in cell culture media, as it is able to both accept and donate protons, thereby regulating pH changes. Its chemical structure and properties make it highly effective in maintaining a constant and optimal pH for many biological and enzymatic reactions. HEPES is commonly used in cell culture, protein purification, and molecular biology techniques.
Application
HEPES has several applications in various research fields. Some of the common applications of HEPES are:
Cell culture: HEPES is commonly used as a component in cell culture media to maintain a stable pH environment. It helps regulate pH changes caused by metabolic activities and the accumulation of waste products in cell cultures.
Enzyme assays: HEPES is often utilized as a buffering agent in enzyme assays to maintain a constant pH, which is crucial for the proper functioning of enzymes. It helps ensure accurate and reproducible results.
Electrophoresis: HEPES is used as a buffering agent in electrophoresis techniques like polyacrylamide gel electrophoresis (PAGE) and agarose gel electrophoresis. It helps maintain the desired pH range for optimal separation and analysis of macromolecules such as proteins and nucleic acids.
Protein purification: HEPES is sometimes included in protein purification buffers to maintain the desired pH during different steps of the purification process. It helps maintain the stability and activity of proteins during the purification steps.
Molecular biology techniques: HEPES can be used in various molecular biology techniques, such as PCR (polymerase chain reaction) and DNA sequencing, to maintain a stable pH. It helps optimize the performance of enzymes and ensures accurate and reliable results.
It is important to note that the specific concentration of HEPES and its application may vary depending on the experimental conditions and requirements.
