Products
4-Morpholineethanesulfonic acid
Synonyms:2-(N-morpholino)ethanesulfonic acid;2-(N-morpholino)ethanesulfonic acid, sodium salt;2-morpholinoethanesulfonate;4-morpholineethanesulfonate;MES compound
Chemical Property of 3-Nitrobenzaldehyde
- Appearance/Colour:White/clear crystalline powder
- Melting Point:>300 °C(lit.)
- Refractive Index:1.525
- Boiling Point:102°C
- PKA:pKa at 0.1M ionic: 0°, 6.38; 20°, 6.15; 37°, 5.98; pKa1 1.99; pKa2 6.21; pKa/°C -0.011
- PSA：75.22000
- Density:1.349 g/cm3
- LogP:0.22510
- Storage Temp.:Store at RT.
- Solubility.:H2O: 0.5?M at?20?°C, clear
- Water Solubility.:185.2g/L at 20℃
- XLogP3:-3.5
- Hydrogen Bond Donor Count:1
- Hydrogen Bond Acceptor Count:5
- Rotatable Bond Count:3
- Exact Mass:195.05652907
- Heavy Atom Count:12
Complexity:214
Safty Information
- Pictogram(s):
Xi - Hazard Codes:Xi
- Statements:36/37/38
- Safety Statements:S22:; S24/25:;
Useful
- Chemical Classes:Other Uses -> Biological Buffers
- Canonical SMILES:C1COCCN1CCS(=O)(=O)O
- Uses:Biological buffer. MES solution has been used to activate microspheres. It has also been used as a component of activating solution to activate carboxyl groups of poly lactic-co-glycolic acid (PLGA).
uses
4-Morpholineethanesulfonic acid (MES) is a commonly used buffer in biochemical research and molecular biology. Here are some key points about MES:
Buffer: MES is used as a buffering agent to maintain a constant pH in biological and chemical experiments. It has a pKa of approximately 6.15, making it effective for maintaining a pH in the range of 5.5 to 6.7.
Stability: MES has good stability at various temperatures and is particularly useful for maintaining pH in the physiological range. It is less affected by temperature changes compared to other buffers like phosphate buffers.
Protein and enzyme studies: MES is commonly used in protein purification, enzyme assays, and other biochemical experiments involving proteins and enzymes. Its low UV absorbance at commonly used wavelengths makes it suitable for spectrophotometric measurements.
Cell culture: MES is also used in some cell culture media to help maintain a stable pH for the growth and maintenance of certain cell types.
pH range: MES is most effective at pH values around 6.0. It is less suitable for applications that require a more acidic or alkaline pH.When working with MES, it is essential to carefully follow the instructions provided by the manufacturer, including the appropriate concentration and pH required for specific applications.
It is also important to note that MES can be irritating to the eyes, skin, and respiratory tract, so appropriate precautions and safety measures should be taken when handling this compound.
