Products
3-(N-Morpholino)propanesulfonic acid hemisodium salt
Synonyms:MOPS HEMISODIUM IN FOIL POUCHES,*TRU-MEA SURE CHEMIC;MOPS, hemisodium salt 3-(N-Morpholino)propanesulfonic acid, hemisodium salt
Chemical Property of 3-(N-Morpholino)Propanesulfonic Acid Hemisodium Salt
● PKA:7.2(at 25℃)
● PSA：153.27000
● LogP:0.88780
● Storage Temp.:Store at RT.
● Solubility.:H2O: 0.5 g/mL, clear, colorless
Safty Information
● Pictogram(s):
Xi
● Hazard Codes:Xi
● Statements:36/37/38
● Safety Statements:22-24/25-36-26
Detailed Introduction
3-(N-Morpholino)propanesulfonic acid hemisodium salt, commonly referred to as MOPS-Na, is a chemical compound that serves as an important buffering agent in various biological and molecular biology applications. The compound consists of a propane chain with a morpholine group attached to the third carbon and is a sulfonic acid derivative.
MOPS-Na is widely used due to its ability to maintain a stable pH in solutions. It is particularly valuable in research involving pH-dependent enzymatic reactions and cell culture systems. MOPS-Na can effectively maintain pH levels within a specific range and minimize fluctuations, ensuring optimal experimental conditions.
One of the notable features of MOPS-Na is its biological compatibility. It is minimally toxic to most organisms, making it suitable for use in cell culture media and other biological systems where maintaining cell viability is essential.
The hemisodium salt form of MOPS-Na refers to the presence of one sodium ion per molecule of MOPS. This salt form increases the compound's solubility and enhances its buffering capabilities.
MOPS-Na is commonly used in the preparation of electrophoresis buffers, including Tris-MOPS-SDS, which is frequently employed in protein molecular weight determination by SDS-PAGE. Additionally, it is utilized in molecular biology techniques such as DNA and RNA gel electrophoresis, as well as in the synthesis of nucleotides and oligonucleotides.
In summary, MOPS-Na is an essential compound in the field of biological research and molecular biology, primarily serving as a buffering agent to maintain stable pH conditions. Its compatibility with biological systems, low toxicity, and role in various experimental techniques make it a valuable tool for scientists studying cellular processes and biomolecules.
Application
3-(N-Morpholino)propanesulfonic acid hemisodium salt (MOPS-Na) is commonly used as a buffering agent in various biological and biochemical applications. Its main application is in molecular biology research, particularly in the following areas:
Cell culture and media: MOPS-Na is often added to cell culture media to maintain a stable pH, ensuring optimal conditions for cell growth and viability. It helps regulate pH changes caused by cellular metabolism and carbon dioxide levels.
Electrophoresis buffers: MOPS-Na is frequently used in gel electrophoresis techniques, such as SDS-PAGE and agarose gel electrophoresis. It is an essential component in running buffers used for separating proteins, DNA, and RNA based on their size and charge.
Enzyme assays: MOPS-Na is used as a buffering agent in enzymatic reactions because it maintains a constant pH over a wide range of temperatures. This allows researchers to accurately measure enzyme activity and kinetics.
Biochemical reactions: MOPS-Na is employed in various biochemistry and molecular biology assays, such as protein purification, gene expression, and enzyme characterization. It helps stabilize reaction conditions, particularly in pH-sensitive reactions.
Nucleotide and oligonucleotide synthesis: MOPS-Na is used as a buffer in the synthesis and purification of nucleotides and oligonucleotides. It helps maintain an optimal pH during synthesis and ensures the stability of these biomolecules.
Polymerase chain reaction (PCR): MOPS-Na can be used as a buffer in PCR amplification, particularly for certain applications requiring specific pH conditions.
Overall, MOPS-Na's buffering properties make it a valuable tool in various biological and biochemical experiments that demand precise pH control. Its applications range from cell culture and molecular biology techniques to protein purification and enzyme characterization.
