Products
3-Morpholinopropanesulfonic acid
Synonyms:3-morpholin-4-ium-4-ylpropane-1-sulfonate;CHEBI:39076;3-(N-morpholiniumyl)propanesulfonate;AKOS015962108;3-(4-morpholin-4-iumyl)-1-propanesulfonate;A803035
Chemical Property of 3-Morpholinopropanesulfonic Acid
● Appearance/Colour:white powder
● Vapor Pressure:0Pa at 25℃
● Melting Point:277-282 ºC
● Refractive Index:1.512
● PKA:7.2(at 25℃)
● Flash Point:116 ºC
● PSA：75.22000
● Density:1.298 g/cm3
● LogP:0.61520
● Storage Temp.:Store at RT.
● Solubility.:H2O: 1 M at 20 °C, clear
● Water Solubility.:1000 g/L (20 ºC)
● XLogP3:-3.2
● Hydrogen Bond Donor Count:1
● Hydrogen Bond Acceptor Count:4
● Rotatable Bond Count:3
● Exact Mass:209.07217913
● Heavy Atom Count:13
● Complexity:214
Safty Information
● Pictogram(s):
Xi
● Hazard Codes:Xi
● Statements:36/37/38
● Safety Statements:26-36
Useful
Canonical SMILES:C1COCC[NH+]1CCCS(=O)(=O)[O-]
Description:MOPS (3-morpholinopropanesulfonic acid) is a buffer introduced by Good et al. in the 1960s. It is a structural analog to MES. Its chemical structure contains a morpholine ring. HEPES is a similar pH buffering compound that contains a piperazine ring. With a pKa of 7.20, MOPS is an excellent buffer for many biological systems at near-neutral pH.It is used as a synthetic buffering agent below pH 7.5.
Uses:3-(N-Morpholino)propanesulfonic acid or MOPS due to its inert nature is a preferred and widely used buffer in many biochemical studies.MOPS has been used as:a cell culture additive component in lentiviral particle production.as a buffering agent in microbial growth medium and nuclei extraction buffer.as a component of Roswell Park Memorial Institute (RPMI) medium for diluting fungal inoculum.as a buffer in capillary-zone electrophoresis to test performance.for the dilution of proteins from algal samples. MOPS acts as a multi-purpose buffering agent used in various biological research. MOPS has been used as:a cell culture additive component in lentiviral particle productionas a buffering agent in microbial growth medium and nuclei extraction buffer
Detailed Introduction
3-Morpholinopropanesulfonic Acid, also known as MOPS, is a chemical compound commonly used as a buffering agent in various biological and biochemical applications. It is a white crystalline powder that is soluble in water.
MOPS has a chemical formula of C7H15NO4S and a molecular weight of 209.26 g/mol. It has a pKa value of approximately 7.2, making it an effective buffer for maintaining physiological pH around 7.0-7.5.
One of the main uses of MOPS is in molecular biology and biochemistry research to regulate pH in various biological assays and experiments. It is commonly used in applications such as DNA/RNA isolation, protein extraction, enzymatic reactions, and gel electrophoresis. MOPS provides stable and consistent pH control, allowing researchers to maintain optimal conditions for their experiments.
MOPS can also be used as a buffer in cell culture media and in chromatography techniques. It is known for its low ultraviolet (UV) absorbance in the range of 240-300 nm, making it suitable for use in UV-based applications.
When working with MOPS, it is important to handle it with proper care. It should be stored in a cool, dry place and kept away from incompatible materials. Safety precautions, such as wearing appropriate protective equipment, should be followed when handling this compound.
As with any chemical, it is advisable to review the safety data sheet (SDS) and consult with the manufacturer or a qualified professional for specific handling and usage instructions.
Application
3-Morpholinopropanesulfonic Acid (MOPS) has a variety of applications in different fields. Some common applications include:
Buffering Agent: MOPS is frequently used as a buffering agent in biological and biochemical experiments. It helps maintain a stable pH in solutions, especially in the physiological pH range of 7.0-7.5
Molecular Biology and Biochemistry: MOPS is widely used as a buffer in DNA/RNA isolation, protein extraction, enzymatic reactions, and gel electrophoresis. It provides optimal pH conditions for these assays and ensures reliable results.
Cell Culture Media: MOPS is utilized as a buffering agent in cell culture media formulation. It helps maintain the pH within the desired range, providing a stable environment for cell growth and viability.
Chromatography: MOPS is used as a buffer in various chromatography techniques, including ion exchange chromatography and size exclusion chromatography. It ensures consistent pH conditions during separation and purification processes.
Chemical Synthesis: MOPS can be employed as a reagent or solvent in chemical synthesis reactions. Its stability, solubility, and pH buffering properties make it suitable for various synthetic applications.
UV Spectroscopy: MOPS has low absorption in the UV range between 240-300 nm, making it useful in UV spectroscopy experiments. It allows accurate measurements without interference from the buffer itself.
These are just a few examples of the many applications of 3-Morpholinopropanesulfonic Acid (MOPS). Its versatility and pH control properties make it a valuable tool in various scientific and research settings.
