3-[Tris-(hydroxymethyl)-methylamino]-1-propanesulfonic acid

TAPS, also known as N-Tris(hydroxymethyl)methyl-3-aminopropanesulfonic acid, is another buffering agent commonly used in biochemical and biological research. Here are some key points about TAPS:
Buffering properties: TAPS is an effective buffering agent that can maintain a pH range of 7.7-9.1. Its pKa value is 8.46, which makes it suitable for buffering solutions within this pH range.
Stability: TAPS is known for its good stability over a wide range of temperatures, making it useful in experiments that require precise pH control. It is less affected by temperature changes compared to other buffering agents like Tris and phosphate buffers.
Biological applications: TAPS is commonly used in various biological and biochemical experiments, such as protein purification, enzyme assays, and DNA/RNA isolation. Its good buffering capacity and stability at physiological pH make it applicable in these contexts.
Cell culture: TAPS can also act as a buffering agent in cell culture media. Its buffering capacity helps in maintaining the desired pH environment for cell growth and maintenance.
Compatibility and ions: Like EPPS, TAPS is a zwitterionic compound, meaning it contains both positive and negative charges. This characteristic makes it effective for buffering both acidic and basic solutions. TAPS is compatible with various biological molecules and has minimal interference with enzymatic reactions.
When using TAPS, it is important to follow the recommended concentration and pH guidelines provided by the manufacturer. Always practice proper safety precautions when handling any chemical or buffering agent.