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2-[Tris(hydroxymethyl)methylamino]-1-ethanesulfonic acid
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Synonyms:CHEBI:39036;2-{[2-hydroxy-1,1-bis(hydroxymethyl)ethyl]ammonio}ethanesulfonate;n-tris(hydroxymethyl)methyl-2-aminoethanesulfonate;N-tris(hydroxymethyl)methyl-2-ammonioethanesulfonate
Chemical Property of 2-[Tris(hydroxymethyl)methylamino]-1-ethanesulfonic acid
● Appearance/Colour: White crystalline powder
● Melting Point: ~223-225 °C
● Refractive Index: 1.57
● PKA: 7.5(at 25℃)
● PSA: 135.47000
● Density: 1.554 g/cm3
● LogP: -1.34880
● Storage Temp.: Store at RT.
● Solubility.:H2O: 1 M at 20 °C, clear, colorless
● Water Solubility.: SOLUBLE
● XLogP3: -5.8
● Hydrogen Bond Donor Count: 4
● Hydrogen Bond Acceptor Count: 6
● Rotatable Bond Count: 6
● Exact Mass: 229.06200837
● Heavy Atom Count: 14
● Complexity: 220
Safty Information
● Pictogram(s):
Xi
● Hazard Codes:Xi
● Statements:36/37/38
● Safety Statements:22-24/25-36-26
Useful
● Canonical SMILES: C(CS(=O)(=O)[O-])[NH2+]C(CO)(CO)CO
● Uses: A structural analog to Tris buffer. Biological buffer. TES is a commonly used buffering agent.
Detailed Introduction
2-[Tris(hydroxymethyl)methylamino]-1-ethanesulfonic acid, also known as TES or N-2-Hydroxyethylpiperazine-N'-2-ethanesulfonic acid, is a zwitterionic buffering compound commonly used in biochemical and molecular biology research.
TES is often employed as a buffer in laboratory experiments due to its ability to maintain a stable pH environment, particularly in the range of 6.5 to 8.5. It also possesses low ultraviolet light absorption, which makes it suitable for use in spectrophotometric applications.This compound is inert and does not interfere with many enzymatic reactions, allowing for reliable experiments.
TES is commonly used in biological and biochemical assays, protein purification, electrophoresis, and cell culture media.
It is worth noting that TES is hygroscopic, meaning it readily absorbs moisture from the environment, so it is crucial to store and handle it properly to maintain its effectiveness.
Application
2-[Tris(hydroxymethyl)methylamino]-1-ethanesulfonic acid (TES) is a useful compound primarily known for its buffering properties. Some of its key uses are:
Buffering agent: TES is commonly used as a buffering component in biochemical and molecular biology experiments. It helps to maintain a stable pH environment, particularly in the range of 6.5 to 8.5
Biological and biochemical assays: TES is utilized in various biological and biochemical assays where maintaining a specific pH for optimal results is crucial. It helps to maintain the pH of the assay solution and provides stability to the reaction conditions.
Protein purification: TES is often used in protein purification processes due to its buffering capacity and compatibility with enzyme activities. It helps to maintain the stability and activity of proteins during purification steps.
Electrophoresis: TES is employed as a buffering component in gel electrophoresis techniques, particularly in polyacrylamide gel electrophoresis (PAGE). It provides a stable pH environment for protein separation and migration.
Cell culture media: TES is included in cell culture media to maintain a constant pH for optimal cell growth and proliferation. It helps to stabilize the pH of the culture medium and provide a suitable environment for the cells.
It is essential to note that the specific application and concentration of TES may vary depending on the experimental requirements. Proper handling, storage, and attention to details outlined in the product's technical documentation are important to ensure its effectiveness and safety.
